or the story of bugs-in-a-bug (bacteria in/on an insect)
Figure 1: aphid feeding, mouthpart showing (stylets in the furrow)
The EPI-Cuticle group-project is a biomimicry-based multidisciplinary project on insect cuticle. It started from scratch early 2016 and comprises people from three labs on the LyonTech Campus : IMP UMR5223 (Polymers, PI Laurent David), LaMCoS UMR5259 (Mechanics, PI Aline Bel-Brunon) & MAP UMR5240 (Microbiology, and Insect Science PI Yvan Rahbé). Within each participating research unit, other researchers are involved: IMP (Pierre Alcouffe, Mathieu Fumagalli, Guillaume Sudre, Stephane Trombotto), LamCoS (Andrea Bareggi) and MAP (Natalia Guschinskaya, Guy Condemine).We are welcoming other people, probably from materials sciences (Mateis) or from nanosciences (INL), in the near future (INL: David Albertini...).
More specifically, we are interested in insect mouth-parts (see below).
You may therefore call us the first insect dentists ever... (Still questionning the link with microbiology ? see this )
Please follow one of our web topics reviews on that matter...
Our group belongs to the newly funded CNRS GDR Biomim, network (GDR2088 BIOMIM).
EPI-Cuticle group-project (Biomimicry)
EPI stands for Interdisciplinary group-project (french Equipe-Projet Interdisciplinaire), and is meant to gather, in a single environment, researchers from different laboratories and disciplines, willing to work together on a single biological object (in our case the insect cuticle).
Insect cuticle is a biological polymer assemblage/ alloy composed of four biochemical compartments : the first two, chitin and cuticular proteins, are the core-polymeric objects of our projects. Its chitin component is composed of spatially structured and partially crystalline nanofibers of chitin/chitosan domains. Its protein component is constituted of a few to a hundred individual proteins from a handful families, individually binding or not to chitin, and giving the final assemblage many of its specific properties, either mechanical or biological. The third important part of the biomaterial is its biological glu-component, comprising the aromatic-based cross-linking material of the insect cuticle (the so-called sclerotizing component). Finally, lipids and lipid impregnation of the material makes the final assembly part, but will not be considered extensively in our project. Lipids have important consequences in both the water-barrier behavior and the volatile-based chemical ecology of the insect cuticle, but such properties are studied by other groups and projects.
Insect cuticle is one part and example of the exoskeleton solution adopted by evolutionary successful animal classes within the arthropods (insects, crustaceans, arachnids…), and is challenging the endoskeleton-based solution of the vertebrate sub-phylum (based on different types of solid materials, such as bone -for the skeleton-, and dentine or enamel -for the mouth-parts-).
Figure 2: Xylella fastidiosa — µ-localised biofilm of the bacterial species Xylella fastidiosa in its plant hopper’s mouth. © Almeida group, Berkeley University
As for any other biological solids, insect cuticle bears different peculiarities and functions that deserve specific scientific attention: their biogenesis (development), their morpho-mechanical properties (biomechanics), and their biological adhesion properties (biocommunication). Being externally exposed, the latter property is especially important for the signalling/barrier properties of the arthropod individual with its environment, including its microbial environment.
As for the vertebrate tooth system, the solid cuticle of insect mouth-parts is often hyper-specialized into both extraordinary bio-materials and exceptional biological objects : the butterfly spiral trumps, the fly pads, the coleopteran jaws or the diverse (evolutionary independent) sucking mouth-parts of many insect orders (mosquitoes, plant bugs, lice, fleas etc…) are just examples of an impressive diversity, all deserving specific evo-devo approaches to understand their respective macro-evolutionary scenarii…
In this context, our biological object model of insect cuticle is that of the hemipteran mouthparts (i.e. plant bug), particularly the four internal parts of the Hemipteran stylet, and more specifically that of the aphid stylet.
The biomimetic object is that of a biosyringe, that bears both very peculiar mechanical properties (micro-drilling machine) and biological adhesion properties (microbial filter).
If ever you were not convinced that insect cuticle, and its mouthparts avatars, can do extraordinary things, take a look below...