Offre Post-Doc à MAP 09/2023

lien Web: (à venir) 09/2023

General information

Reference : UMR5240-MAP
Workplace : Villeurbanne
Date of publication : July, 2023
Type of Contract : Fixed-Term Contract Scientist
Contract Period : 30 months 
Expected date of employment : October 2023 to January 2024
Proportion of work : Full time
Remuneration : 2600-3000 euros gross/month depending on experience 
Desired level of education : PhD
Background: molecular biology, microbiology, chromosome/chromatin biology

Missions

We are looking for a postdoctoral researcher interested in the relation between the chromosome structure and expression in the context of bacterial pathogenicity. He/she will work within the CAGE project funded by the ANR in the team Chromatin and Regulation of Bacterial Pathogenicity of the MAP laboratory(https://map.insa-lyon.fr/). The objective is to characterize the chromosome dynamics of the phytopathogen Dickeya dadantii under stress conditions and during plant infection using Hi-C and ChIP-Seq together with transcriptomics, in order to unveil novel mechanisms of virulence regulation, with a particular focus on the activity of Nucleoid Associated Proteins (NAPs) and DNA supercoiling. 

Skills

- Rigor and autonomy
- Experience in bacterial genetics, microscopy, RNA-Seq, ChIP-Seq, or Hi-C (in bacteria or eukaryotes).

- Experience in Next Generation Sequencing analysis (Python language, UNIX environment is preferred; training can be taken).
- Ability to work as a team and be a team player
- Assemble experimental results and ability to present his/her work orally and in writing
- Good English skills 

Work Context

The postdoc will have the opportunity to work in an internationally recognized team in the field of regulation of bacterial virulence in relation to chromosome structure. He/she will develop autonomous research under the supervision of the coordinator of the project, and will benefit of the collaboration with other team members for experimental design and computational analysis and modeling. All equipments needed for the project are available in the host Institute.

The postdoc will also work in close collaboration with Axel Cournac in the group of Romain Koszul, Pasteur Institute, team Spatial Regulation of Genomes, CNRS UMR3525 https://research.pasteur.fr/fr/team/spatial-regulation-of-genomes/

The MAP UMR5240 is a University of Lyon I/INSA-Lyon/CNRS research unit located in the research campus of La Doua (Lyon). The campus is in close proximity to a natural park and is deserved by public transportation with direct access to Lyon city center. The institute hosts around 70 people among 5 research teams and benefits from state-of-the-art technical facilities of FR BioEEnvis http://bioenvis.universite-lyon.fr/-La-Federation-.html

Send CV and motivation letter to: William.nasser@insa-lyon.fr

Selected references: 

- Reverchon S, Meyer S, Forquet R, Hommais F, Muskhelishvili G, Nasser W. (2021) The nucleoid-associated protein IHF acts as a “transcriptional domainin” protein coordinating the bacterial virulence traits with global transcription. Nucleic Acids Res. 

- Pineau, M., Martis S., Forquet R., Baude J., Villard C., Grand L., Popowycz F., Soulère L., Hommais F., Nasser W., Reverchon S., Meyer S.(2022) What is a supercoiling-sensitive gene? Insights from topoisomerase I inhibition in the Gram-negative bacterium Dickeya dadantii. Nucleic Acids Res, 50, 9149-9161.

- Lioy, V.S., Cournac, A., Marbouty, M., Duigou, S., Mozziconacci, J., Espeli, O., Boccard, F. and Koszul, R. (2018) Multiscale Structuring of the E. coli Chromosome by Nucleoid-Associated and Condensin Proteins. Cell, 172, 771-783.e718.

- Matthey-Doret, C., Baudry, L., Breuer, A., Montagne, R., Guiglielmoni, N., Scolari, V., Jean, E., Campeas, A., Chanut, P.H., Oriol, E. et al. (2020) Computer vision for pattern detection in chromosome contact maps. Nat Commun, 11, 5795.

- Muskhelishvili G, Forquet R, Reverchon S, Meyer S, Nasser W. (2019) Coherent domains of transcription coordinate gene expression during bacterial growth and adaptation. Microorganisms 7 (12), pii: E694

- Forquet R, Jiang X, Nasser W, Hommais F, Reverchon S, Meyer S (2022). Mapping the Complex Transcriptional Landscape of the Phytopathogenic Bacterium Dickeya dadantii. mBio. 13(3):e0052422

- Jiang X., Sobetzko P., Nasser W., Reverchon S., and Muskhelishvili G. (2015) Chromosomal "stress-response" domains govern the spatiotemporal expression of the bacterial virulence program. Mbio 6: e00353-15